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Title: Development, standardisation and validation of purine excretion technique for measuring microbial protein supply in yerli kara cross breed
Authors: Çetinkaya, Nurcan
Yaman, S.
Özdemir, Hülya
Gücüş, Ali İhsan
Özcan, Hüseyin
Söğüt, Ali
Keywords: Yerli kara cross breed
Yerli kara melezi
Microbial protein supply in
Mikrobiyal protein arzı
Purine excretion technique
Pürin atılım tekniği
Standardisation and validation
Standardizasyonu ve validasyon
Issue Date: 2003
Publisher: National Nuclear Center of the Republic of Kazakhstan / Institute of Nuclear Physics
Citation: Çetinkaya, N. ... [ve arkadaşları]. (2003). Development, standardisation and validation of purine excretion technique for measuring microbial protein supply in yerli kara cross breed. The Second Eurasian Conference on Nuclear Science and Its Application : Presentations, 3.c., (s.64-75). 16-19 September 2002. Almaty : Kazakhstan.
Abstract: Three experiments were conducted to evaluate of the developed techniques for uric acid, allantoin and creatinine in Yerli Kara cross-breed cattle on farm at different feeding level locally available feed resources and linking the observed information to feed intake and to assess of protein nutrition status of Yerli Kara cross-breed dairy cattle using urinary PD and creatinine excretion. In Experiment I. Response of daily PD excretion to feed intake in Yerli Kara crossbreed on state farm was measured. Animals were fed a mixed diet containing 30% wheat straw and 70% compounded feed. The diet contained 90% DM, its N and OM contents were 124 and 950 g/kg DM, respectively. In Experiment II. Spot urine sampling techniques was applied at state farm. Four Yerli Kara cross-breed bulls live weight with a mean of 211±41.3 kg were used. Experimental design, feeding and diet were the same as in Experiment I. The treatments were allocated according to a 4x4 Latin Square design. In Experiment III. Spot urine sampling techniques was applied at smallholder farms. Compound feed containing 65% barley, 25% bran, 6% sunflower seed meal, 3% marmer dust and 1% mineral and vitamin mixture (120 g/kg DM-Crude Protein and 950 g/kg DM-Organic Matter)- was offered total in between 2 to 3 kg in two parts one in the morning (07:30 h) and one in the afternoon (17:00 h). Compound feed ingredients were similar given to all animals but Groups I, II and III animals were receiving 1 to 2 kg/d of straw (30 g CP/kg DM, 930g OM/kg DM), grass hay (70g CP/kg DM ,915 g OM/kg DM), straw and grass hay respectively. There were significant correlations (R²=0.99) between PD excretion (mmol/d) and DOMI (kg/d) for YK-C cattle. PD exceretion (mmol/d) was plotted against PD: Creatinin W0.75 to obtain slope and use as constant for the estimation of daily PD exceretion from spot sampling from animals held by small holders. The equation could be expressed as: PD (mmol/d)= -2.3+0.953 ([PD]:[C]xW0.75). The constant (C)-the slope as mmol/kg W0.75 was 0.953.The coefficient of variation (CV) for the uric acid, allantion, PD, creatinine, total-N, PD:CxW0.75 (PDC Index) in spot urine samples of four treatments were less than 5%. Developed banding system can be used for YK and YK-C cattle for the spot urine sample measurement.There were not significant difference for each parameter between the spot urine sampling times of Groups I, II and III. The corresponding microbial-N values to PDC Index of groups I. II and III were 15-25 g/d. Experimentaly estimated DOMI was 2.21±0.15 kg/d. By using the equation (DOMI= 344+48.7xPDC Index) estimated DOMI (g/d) of groups I, II, and III were 2.8±0.6, 2.6±0.7 and 2.7±0.7 respectively. CV% of Groups I, II and III were 22, 27 and 26% respectively. In conclusion, the PDC Index in spot urine samples could be used under farm condition as an indicator of microbial protein supply in YK-C cattle, and also DOMI can be estimated from PDC Index in spot urine samples under field conditions.
Description: TENMAK D.N.3328
Appears in Collections:II. Eurasian conference nuclear science and its application : proceedings, 16-19 September, 2002. Almaty, Kazakhstan

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