Türkiye Enerji, Nükleer ve Maden Araştırma Kurumu Kurumsal Araştırma Arşivi

Kurum yayınları ve kurum çalışanlarının yayınları “Açık Erişim” kapsamında yayınlanmaktadır. Açık Erişim “evrensel nitelikteki bilimsel bilginin insanlığın yararına sunulması” amacını taşır. Tezler, konferans bildirileri ve sunumlar ile makaleler kurum çalışanları tarafından sisteme girilmektedir.Tezler için; YÖK'ten alınan görüş doğrultusunda tez sahibinin izin vermesi yeterlidir. Konferans bildirileri ve sunumların yayına gönderildiği son hali arşive konulabilir.Makaleler için ise; hakem onayından geçmiş, yayına gönderildiği son halinin (post-print) Açık Erişime sunulmasına yayıncılar %90 oranında izin vermektedir. Arşivde şu an 1.639 yayın bulunmaktadır, çalışma bitiminde bu sayı yaklaşık 2.000 yayın sayısına ulaşacaktır.

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Impact of low energy proton radiation on lentil seeds (Lens culinaris) for sustainable space breeding

(ELSEVIER, 2025-10-26) Fırat, Suna; Opçin, Büşra; Yıldırım. Aydın

The effects of low energy proton irradiation on lentil (Lens culinaris) seeds were investigated to evaluate their potential for space-based breeding. Seeds of a local cultivar were exposed to 2.28 MeV protons for 5 min at beam currents ranging from 50 to 225 nA, corresponding to absorbed doses of 8.45–38.02 kGy, alongside a nonirradiated control group. Germination was assessed on day 5, and survival rate, seedling height, first true leaf length, and biomass were measured after 27 days of greenhouse growth. Linear energy transfer (LET) analysis using SRIM indicated that maximum energy deposition occurred within the outer cotyledon layers. Beam currents up to 75 nA showed no significant differences from the control in germination or survival, whereas currents above 150 nA markedly suppressed growth parameters, with survival reduced by up to 92.5% at 225 nA. As observed in studies with other ionizing radiation types, low current, low energy exposures produced no adverse effects, suggesting that lentil seeds may tolerate low-dose proton irradiation. These results highlight the importance of radiation shielding in deep-space missions and support the use of lentils as a candidate crop for controlled breeding systems in extraterrestrial environments.

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Development of Petal Culture Method in Kalanchoe (Kalanchoe blossfeldiana Poelnn.) and Investigation of its Potential Use in In Vitro Mutation Breeding Studies

(2025-07-01) Kantoğlu Kadriye Yaprak; Sarıtoprak Okan; Akyüz Çağdaş Ebru; Okutan Evrim; Aktaş Hakan; Ellialtıoğlu Şeküre Şebnem

Kalanchoe (Kalanchoe blossfeldiana Poelnn.), is an important potted indoor plant as well as an outdoor ornamental plant and cut flower in recent years. Studies are being carried out using different breeding methods in order to develop new varieties suitable for market needs. In vivo and in vitro mutation breeding studies are important for creating different genetic variations for this species, which is highly prone to mutation breeding. In in vitro mutation applications, vegetative propagation methods are of critical importance in mass propagation of mutant single individuals while preserving genetic stability. This study consists of two stages: First, development and optimization of petal culture method in kalanchoe to provide in vitro vegetative propagation of mutant individuals, and second, morphological observation of genetic stability in clones obtained by propagation via petal culture from M1V4 kalanchoe mutant single plants obtained by using ionizing radiation. In vitro petal culture conditions were determined for kalanchoe and it was determined that Murashige and Skoog (MS) nutrient medium containing 2.0 mg L-1 thidiazuron (TDZ), 0.5 mg L-1 1-naphthalenacetic acid (NAA), 30 g L-1 sucrose, 6 g L-1 agar and pH 5.7 provided the best regeneration. In addition, after in vitro physical mutagen application, flowers were observed in mutant individuals propagated up to M1V4 stage in laboratory conditions and transferred to external conditions. Petals from plants with 4 different mutant flowers selected from these were cultured and propagated in vitro. As control, petals from a commercial variety were used for micropropagation. The flowers of the clones obtained showed homogeneity depending on whether the mutant flowers used as starting material were homogeneous or chimeric in appearance. Following this study, in which the first findings on petal culture in kalanchoe were obtained, studies are continuing to develop it comprehensively.

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Fraser Photinia shoot explantation in vitro: Effects of two distinct gammaray sources and identification of the optimal mutation dose

(T.C. Türkiye Enerji, Nükleer ve Maden Araştırma Kurumu, 2025-06-25) Türkmen Onur Sinan; Kantoğlu Kadriye Yaprak; Ellialtıoğlu Şeküre Şebnem

Because of its beautiful qualities and ability to withstand harsh conditions, Fraser photinia (Photinia × fraseri cv. Red Robin) is frequently used as an ornamental plant in garden designs. The efforts to create new, highly marketable variations of the species have begun to increase in response to the growing ability of the current kinds to adapt to changing climatic circumstances. For this species, which is susceptible to in vitro propagation, the in vitro mutation breeding technique holds significant promise for increasing the current variety. It is essential to ascertain whether ionizing gamma ray sources are suitable for in vitro mutation investigations on Fraser photinia. To achieve this, in vitro shoot explants were exposed to a total of thirteen different radiation doses using 60Co (dosage rate: 235 Gy/h) and 137Cs (dosage rate: 821 Gy/h) gamma ray sources. The number of leaves and shoot length in in vitro plantlets were assessed thirty days after irradiation, and linear regression analysis was used to get the effective mutation dose (EMD50) values. Based on the quantity of leaves, the EMD50 for the 137Cs source ray application was 60.34 Gy, whereas the 60Co source resulted in an EMD50 of 80.88 Gy. These findings demonstrated that the EMD50 difference was significantly impacted by the source power, irradiation duration, and the influence produced by the linear energy transfer value of the irradiation during tissue penetration.

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DETERMINING THE EFFECTIVE MUTATION DOSE AND IMPACTS OF IRRADIATION FOR SOME VEGETABLE SPECIES

(Biotechnical Faculty of the University of Montenegro, 2025-07-01) Kantoğlu Kadriye Yaprak; Çakın Irmak; Çetintaş Aydın Ozan; Göktuğ Aslıhan; Laouini Mouna

A comparison of winter and summer vegetable species reveals that fewer mutation breeding studies have been conducted on winter varieties. Nevertheless, the effects of global warming and climate change are expected to negatively impact the cultivation of both winter and summer vegetable species alike. Addressing this issue promptly and developing new cultivars as alternatives to existing ones in accordance with current needs is crucial. Mutation breeding methods remain significant in efforts to broaden the diminishing genetic diversity. In this research, various vegetables, including red radish, black radish, white cabbage, broccoli, cress, dill, yellow onion, purple onion, spinach, parsley, and arugula, were exposed to a cesium 137 gamma ray source. The irradiation was conducted at different dose levels (0, 50, 100, 200, 300, 400, 400, 500, 600, 700, 800, 900, 1000, 1200, 1400, 1600, and 1800 Gy), based on the specific responses of each cultivar. Following irradiation, the seed germination rate, seedling height, seedling fresh and dry weight, and seed vigor were measured, and the effective mutation dose (EMD50) was determined by linear regression analysis. It was found that increasing doses had a negative effect on germination, seed strength, and fresh and dry plant weight, depending on the species. Accordingly, EMD50, which is important in creating an effective mutant population, was determined as 467.62 Gy for red radish, 328.39 Gy for black radish, 354.24 Gy for broccoli, 222.25 Gy for white cabbage, 119.75 Gy for arugula, 160.68 Gy for spinach, 245.96 Gy for yellow onion, 195.55 Gy for purple onion, 132.38 Gy for dill, 1031.68 Gy for cress, and 92.77 Gy for parsley.

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In vitro Mutasyon Islahı Çalışmalarına Yönelik Olarak Farklı Gama Işını Kaynaklarının Kalanşo (Kalanchoe blossfeldiana Poelnn.) Yaprak Ayası Eksplantı Üzerine Etkilerinin Karşılaştırılması

(Dergi Park Yalova Atatürk Bahçe Kültürleri Araştırma Enstitüsü, 2025-01-30) Çakın Irmak; Kunter Burak; Kantoğlu Kadriye Yaprak; Göktuğ Aslıhan; Akyüz Çağdaş Ebru; Ellialtıoğlu Şebnem

Kalanşo (Kalanchoe blossfeldiana Poelnn.) uzun ömürlü iç mekân süs bitkisi türlerinden biri olup in vitro rejenerasyon kabiliyeti yüksektir. Bu araştırmada in vitro mutasyon ıslahı çalışmasına yönelik olarak yaprak ayası eksplantlarında, iki farklı gama ışınlama kaynağında ışınlama yapılarak etkili mutasyon dozlarının belirlenmesi (EMD₅₀) ve kaynak etkisinin ortaya konulması hedeflenmiştir. Bu amaçla her dozda 60’şar yaprak ayası eksplantı olacak şekilde in vitro kültürler on dört farklı dozda (0, 10, 20, 30, 40, 50, 60, 70, 80, 90, 100, 120, 140 ve 160 Gy) Co⁶⁰ (235 Gy/h) ve Cs¹³⁷ (821 Gy/h) gama ışın kaynakları ile ışınlanmıştır. Işınlanan in vitro eksplantlar ışınlama sonrasında rejenerasyon ortamına (2.0 mg.L⁻¹ TDZ ve 0.5 mg.L⁻¹ NAA) transfer edilmişlerdir. Işınlamayı takip eden kırk beşinci günde eksplantlarda sürgün rejenerasyonu başarı yüzdeleri tespit edilerek iki farklı gama kaynağının etkileri karşılaştırılmıştır. In vitro rejenerasyon amacıyla S2 kodlu uygulamanın (%15’lik NaClO içeren sterilizasyon çözeltisi) ve R3 kodlu 2.0 mg.L⁻¹ TDZ ve 0.5 mg.L⁻¹ NAA içeren MS besin ortamının kullanılabileceği belirlenmiştir. EMD₅₀ dozu olarak; Cs¹³⁷ kaynağı için 79.97 Gy ve Co⁶⁰ kaynağı için ise 103.54 Gy hesaplanmıştır. Bununla birlikte kobalt kaynağı için daha yüksek dozlarda bazı yeni uygulamaların yapılması önerilmiştir.

Türkiye Enerji, Nükleer ve Maden Araştırma Kurumu Kurumsal Araştırma Arşivi

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