Makale

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Browsing Makale by All Authors "176647"

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    Determination of effective mutagen dose for lettuce (Lactuca sativa var. longifolia cv. cervantes) seeds
    (CES Science Group, 2017) Sarıçam, Şule; Kantoğlu, K. Yaprak; Ellialtıoğlu, Ş. Şebnem; 176647; TAEK-SANAEM
    In plant breeding strategies induced mutagenesis has become an effective way of supplementing the existing germplasm and improving new varieties. Mutation can be induced by chemical and physical mutagens or by combination. The commercial importance and production of lettuce, which is the most popular of the local salad crops, is increasing in Turkey. Lettuce is an important vegetable commodity and in demand by the local markets throughout the year. This study was carried out to get database for mutation breeding studies on lettuce cv. Cervantes. For this aim, 0, 50, 100, 200, 300, 400, 500 and 600 Gray (Gy) doses of Co60 (gamma-rays) were treated on lettuce seeds as a physical mutagen. 30 seeds were used for each dose. Thirty days after treatment, germination and shoot developing of cloves were determined. The Effective Mutagen Dose (EMD50) calculated by linear regression analyses. According to results, 372.66 Gy dose was found as EMD50.
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    Tissue culture applications inlettuce (Lactuca sativa L.)
    (CES Science Group, 2017) Sarıçam, Şule; Kantoğlu, K. Yaprak; Ellialtıoğlu, Ş. Şebnem; 176647; TAEK-SANAEM
    Lettuce (Lactuca sativa L.) is a major fresh leafy vegetable and belongs to the Asteraceae family (Compositae). The focus of modern lettuce breeding objectives is to improve horticultural characteristics such as quality, resistance to early bolting and breeding for resistance to pests and diseases. These characteristics may be improved using biotechnology and gene transfer strategies. Tissue culture methods have proved successful in rescuing selected lettuce genotypes and producing seeds in a disease-free environment. And also overcome the limitations of in vivo techniques by shortening period of breeding programmes. The genetic engineering of lettuce requires a reliable and efficient tissue culture methods. Among these methods haploidization is of great interest for producing pure lines. Shoot organogenesis may aid the use of genetic engineering to improve important characteristics of lettuce. Embryo rescue is an important application for transfering the resistance to downy mildew, LMV, and beet western yellows virus from L. virosa and L. saligna into cultivated lettuce. Somatic hybridization via protoplast fusion has also been used to gain access to exotic, sexually incompatible germplasm. Propagation from axillary and apical buds of lettuce plants can also be routinely carried out. If propagation through tissue culture can be done efficiently on a large scale, it may be used to produce F1 hybrid plants and fix the hybrid vigour. Somaclonal variation provides another source of genetic variability. Regeneration via Somatic embryogenesis has advantages for lettuces.