Makale

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Browsing Makale by Organisation Author "Çetintaş, Aydın Ozan"

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    Determination of the Effective Mutation Dose Through Physical Mutagen Application in Pearl Millet (Pennisetum glaucum L. cv. Heavri) for the Mutation Breeding Program
    (Tekirdag Agricultural Faculty, 2026-03-16) Şenay, Ali
    The problems caused by climate change have begun to significantly affect all areas of agricultural production. In particular, the increase in temperature and the resulting drought have become major issues for crop species, leading to an increase in annual yield losses. The advantages of millets (such as their nutritional value and high adaptability) are currently being considered in many countries as an alternative to crops that require more irrigation, especially due to the growing problem of drought. Various methods (classical breeding and biotechnological approaches) are being applied for millet breeding worldwide, and among these techniques, mutation breeding is one method that deserves particular attention. Countries that play an important role in millet production have initiated mutation breeding programs at the national and regional levels to increase nutrient content and enhance disease tolerance. In the present study, the ffective mutation dose was determined as a preliminary step to initiate mutation breeding research for pearl millet (Pennisetum glaucum L. cv. Heavri), which has been agronomically examined in Türkiye. For this purpose, millet seeds with 8% moisture content were irradiated with cesium-137 gamma rays, a physical mutagenic source, at seven different doses (0, 50, 100, 200, 300, 400, and 500 Gray). As a result of the plant measurements taken three weeks after irradiation and the linear regression analysis, 174 Gy was determined to be the effective mutation dose. The negative effects of increasing gamma ray doses on plant development were clearly determined on both shoot length and fresh plant weight, and the resulting difference was determined to be statistically important. The 10% lower and upper limits of the effective mutation dose were defined. Irradiation was conducted at doses of 150, 174, and 200 Gray to generate the M1 generation for the Heavri variety.