Makale

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Browsing Makale by ORCID "000-0001-8886-5348"

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    DETERMINING THE EFFECTIVE MUTATION DOSE AND IMPACTS OF IRRADIATION FOR SOME VEGETABLE SPECIES
    (Biotechnical Faculty of the University of Montenegro, 2025-07-01) Kantoğlu Kadriye Yaprak; Çakın Irmak; Çetintaş Aydın Ozan; Göktuğ Aslıhan; Laouini Mouna
    A comparison of winter and summer vegetable species reveals that fewer mutation breeding studies have been conducted on winter varieties. Nevertheless, the effects of global warming and climate change are expected to negatively impact the cultivation of both winter and summer vegetable species alike. Addressing this issue promptly and developing new cultivars as alternatives to existing ones in accordance with current needs is crucial. Mutation breeding methods remain significant in efforts to broaden the diminishing genetic diversity. In this research, various vegetables, including red radish, black radish, white cabbage, broccoli, cress, dill, yellow onion, purple onion, spinach, parsley, and arugula, were exposed to a cesium 137 gamma ray source. The irradiation was conducted at different dose levels (0, 50, 100, 200, 300, 400, 400, 500, 600, 700, 800, 900, 1000, 1200, 1400, 1600, and 1800 Gy), based on the specific responses of each cultivar. Following irradiation, the seed germination rate, seedling height, seedling fresh and dry weight, and seed vigor were measured, and the effective mutation dose (EMD50) was determined by linear regression analysis. It was found that increasing doses had a negative effect on germination, seed strength, and fresh and dry plant weight, depending on the species. Accordingly, EMD50, which is important in creating an effective mutant population, was determined as 467.62 Gy for red radish, 328.39 Gy for black radish, 354.24 Gy for broccoli, 222.25 Gy for white cabbage, 119.75 Gy for arugula, 160.68 Gy for spinach, 245.96 Gy for yellow onion, 195.55 Gy for purple onion, 132.38 Gy for dill, 1031.68 Gy for cress, and 92.77 Gy for parsley.
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    In vitro Mutasyon Islahı Çalışmalarına Yönelik Olarak Farklı Gama Işını Kaynaklarının Kalanşo (Kalanchoe blossfeldiana Poelnn.) Yaprak Ayası Eksplantı Üzerine Etkilerinin Karşılaştırılması
    (Dergipark, 2025-03-25) Çakın, Irmak; Kunter, Burak; Kantoğlu, Kadriye Yaprak; GÖKTUĞ, Aslıhan; AKYÜZ, Ebru; ELLİALTIOĞLU, Ş.Şebnem
    Kalanşo (Kalanchoe blossfeldiana Poelnn.) uzun ömürlü iç mekân süs bitkisi türlerinden biri olup in vitro rejenerasyon kabiliyeti yüksektir. Bu araştırmada in vitro mutasyon ıslahı çalışmasına yönelik olarak yaprak ayası eksplantlarında, iki farklı gama ışınlama kaynağında ışınlama yapılarak etkili mutasyon dozlarının belirlenmesi (EMD₅₀) ve kaynak etkisinin ortaya konulması hedeflenmiştir. Bu amaçla her dozda 60’şar yaprak ayası eksplantı olacak şekilde in vitro kültürler on dört farklı dozda (0, 10, 20, 30, 40, 50, 60, 70, 80, 90, 100, 120, 140 ve 160 Gy) Co⁶⁰ (235 Gy/h) ve Cs¹³⁷ (821 Gy/h) gama ışın kaynakları ile ışınlanmıştır. Işınlanan in vitro eksplantlar ışınlama sonrasında rejenerasyon ortamına (2.0 mg.L⁻¹ TDZ ve 0.5 mg.L⁻¹ NAA) transfer edilmişlerdir. Işınlamayı takip eden kırk beşinci günde eksplantlarda sürgün rejenerasyonu başarı yüzdeleri tespit edilerek iki farklı gama kaynağının etkileri karşılaştırılmıştır. In vitro rejenerasyon amacıyla S2 kodlu uygulamanın (%15’lik NaClO içeren sterilizasyon çözeltisi) ve R3 kodlu 2.0 mg.L⁻¹ TDZ ve 0.5 mg.L⁻¹ NAA içeren MS besin ortamının kullanılabileceği belirlenmiştir. EMD₅₀ dozu olarak; Cs¹³⁷ kaynağı için 79.97 Gy ve Co⁶⁰ kaynağı için ise 103.54 Gy hesaplanmıştır. Bununla birlikte kobalt kaynağı için daha yüksek dozlarda bazı yeni uygulamaların yapılması önerilmiştir. Kalanchoe (Kalanchoe blossfeldiana Poelnn.) is a long-lived indoor ornamental plant species with high in vitro regeneration ability. The aim of this study was to determine the effective mutation doses (EMD₅₀) in leaf blade explants for in vitro mutation breeding using two different gamma irradiation sources and to identify the source effect. For this purpose, in vitro cultures were irradiated with ⁶⁰Co (235 Gy/h) and ¹³⁷Cs (821 Gy/h) gamma ray sources at fourteen different doses (0, 10, 20, 30, 40, 50, 60, 70, 80, 90, 100, 120, 140, and 160 Gy) with 60 leaf blade explants at each dose. Irradiated in vitro explants were transferred to the regeneration medium (2 mg.L⁻¹ TDZ and 0.5 mg.L⁻¹ NAA) after irradiation. Indirect shoot regeneration success percentages were determined on the forty-fifth day following irradiation, and the effects of the two different gamma sources were compared. S2 (15% NaClO sterilization solution) and R3 (MS nutrient medium containing 2.0 mg.L⁻¹ TDZ and 0.5 mg.L⁻¹ NAA) could be used for in vitro regeneration. The EMD₅₀ dose was calculated as 79.97 Gy for the ¹³⁷Cs source and 103.54 Gy for the ⁶⁰Co source. However, some new applications of higher doses of cobalt sources have been suggested for higher doses of cobalt source.