Makale

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Browsing Makale by Publisher "Biharean Biologist, Oradea, Romania"

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    Characterization of tomato genotypes by simple sequence repeats (SSR) molecular markers
    (Biharean Biologist, Oradea, Romania, 2021-12-05) Aziz, Sibel; Kantoğlu, K. Yaprak; Tomlekova, Nasya; Staykova, Teodora; Ganeva, Daniela; Sarsu, Fatma; https://orcid.org/ 0000-0002-7247-9116; TENMAK-Nükleer Enerji Araştırma Enstitüsü-Ankara
    Microsatellites are highly polymorphic regions containing gene loci represented by multiple alleles of different lengths and nucleotide sequences. The purpose of this study was to identify reproducible specific molecular profiles of tomato varieties and F1 hybrids from the collection of the Maritsa Vegetable Crops Research Institute, Bulgaria, and of mutant lines from the Nuclear Energy Research Institute, Turkey, by detecting polymorphic microsatellite loci as markers. The study included five varieties, four F1 hybrids Bulgarian tomato, five Turkish mutant lines, and an initial landrace analysed with 19 SSR markers located in different chromosomes of the tomato genome at a distance of 6.0 cM to 146.0 cM. Fifteen microsatellite markers were used to generate polymorphic profiles. The number of bands per microsatellite locus ranged from one to eight, with a total of 62 bands among the markers analysed. The average number of SSR alleles per locus was 3.26. The dendrogram constructed with the values of genetic distance showed that all fifteen genotypes are grouped into two major clusters. Twelve form one main cluster with subclusters, while the rest of them form the other cluster. The results of the present study complement the data on the genetic heterogeneity of tomatoes and could be useful for tomato breeding programes.