Makale

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Browsing Makale by Publisher "Biotechnical Faculty of the University of Montenegro"

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    DETERMINING THE EFFECTIVE MUTATION DOSE AND IMPACTS OF IRRADIATION FOR SOME VEGETABLE SPECIES
    (Biotechnical Faculty of the University of Montenegro, 2025-07-01) Kantoğlu Kadriye Yaprak; Çakın Irmak; Çetintaş Aydın Ozan; Göktuğ Aslıhan; Laouini Mouna
    A comparison of winter and summer vegetable species reveals that fewer mutation breeding studies have been conducted on winter varieties. Nevertheless, the effects of global warming and climate change are expected to negatively impact the cultivation of both winter and summer vegetable species alike. Addressing this issue promptly and developing new cultivars as alternatives to existing ones in accordance with current needs is crucial. Mutation breeding methods remain significant in efforts to broaden the diminishing genetic diversity. In this research, various vegetables, including red radish, black radish, white cabbage, broccoli, cress, dill, yellow onion, purple onion, spinach, parsley, and arugula, were exposed to a cesium 137 gamma ray source. The irradiation was conducted at different dose levels (0, 50, 100, 200, 300, 400, 400, 500, 600, 700, 800, 900, 1000, 1200, 1400, 1600, and 1800 Gy), based on the specific responses of each cultivar. Following irradiation, the seed germination rate, seedling height, seedling fresh and dry weight, and seed vigor were measured, and the effective mutation dose (EMD50) was determined by linear regression analysis. It was found that increasing doses had a negative effect on germination, seed strength, and fresh and dry plant weight, depending on the species. Accordingly, EMD50, which is important in creating an effective mutant population, was determined as 467.62 Gy for red radish, 328.39 Gy for black radish, 354.24 Gy for broccoli, 222.25 Gy for white cabbage, 119.75 Gy for arugula, 160.68 Gy for spinach, 245.96 Gy for yellow onion, 195.55 Gy for purple onion, 132.38 Gy for dill, 1031.68 Gy for cress, and 92.77 Gy for parsley.