Effect of radiation on viability and infectivity of nematodirus SP. eggs
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Date
2003
Journal Title
Journal ISSN
Volume Title
Publisher
Turkish Atomic Energy Authority
Abstract
Bu çalışmada, değişik dozlarda ışınlamanın Nematodirus sp. Yumurtalarının açılmasına ve ışınlamanın larvaların enfektivitelerine etkileri ankara keçilerinde araştırılmıştır. Nematodirus sp. Yumurtaları 1-300 Gy arasında değişen dozlarda ışınlanmıştır. Yumurtalardaki larval gelişme incelendiğinde, 1, 5, 10 ve 20 Gy gibi düşük dozlarda ışınlanan gruplarla kontrol grubu arasında yumurtaların açılmasına ilişkin önemli farklar bulunmamıştır. Otuz Gy'lik ışınlamadan sonra, yumurtaların açılma yeteneği ve kullanılan gama radyasyon dozu arasında ters bir ilişki olduğu görülmüştür. Radyasyon dozu arttıkça, daha az sayıda açılma gözlenmiş ve 225 Gy’lik dozdan sonra açılma olmamıştır. Bundan başka, radyasyon dozunun artışına bağlı olarak blastomerlerin çoğalmasında ve gelişmesinde dejeneratif değişiklikler gözlenmiştir. Kontrol ve ışınlama grupları arasındaki fark (30 Gy’den başlayarak) istatistiksel olarak önemli bulunmuştur. Oniki Ankara keçisi, 0, 30, 50 ve 100 Gy dozda ışınlanmış 500’er Nematodirus sp. Larvası ile enfekte edilmişlerdir. Enfeksiyondan 28 gün sonra tüm hayvanlar kesilmiş ve parazit sayımları yapılmıştır. Radyasyon dozunun artışına bağlı olarak yumurta ve parazit sayımlarında bir azalma olduğu belirlenmiş ve 100 Gy dozda ise hiçbir parazit elde edilememiştir. Ayrıca, diğer gruplarda da toplanan parazitlerin çoğunun dişi olduğu görülmüştür.
In this study, effects of different levels of gamma irradiation on the hatching ability of Nematodirus sp. Eggs and infectivity of irradiated larvae in Angora goats were investigated. Nematodirus sp. Eggs were exposed to radiation doses ranging from 1 to 300 Gy. It was fonud that there was no significant differences with respect to hatching ability between the control and irradiated groups of eggs in low doses such as 1, 5,10 and 20 Gy when larval development in eggs were examined. The results show that there is an inverse relatonship between the gamma radiation dose used and the hatching ability of eggs after the dose of 30 Gy. The more the radiation dose level, the lesser the hatching was observed and there was no hatchings after the dose of 225 Gy. Moreover, degenerative changes in the developments and multiplications of blastomrs relating to increases of radiation doses were observed. The differences between the control group and irradiated groups (starting from 30 Gy) were found to be statistically significant (P<0.05). Twelve Angora goats were infected with each receiving 500 Nematodirus sp. Larvae irradiated with doses of 0, 30, 50 and 100 Gy. All animals were sacrificed and worm counts were performed 28 days after infection. It was determined that there were decreases in the egg and worm counts and delayed egg sheddings with the increases of irradiation doses and no worms were obtained at a dose of 100 Gy. Also, the survival of more females than males in the collected parasites was found.
In this study, effects of different levels of gamma irradiation on the hatching ability of Nematodirus sp. Eggs and infectivity of irradiated larvae in Angora goats were investigated. Nematodirus sp. Eggs were exposed to radiation doses ranging from 1 to 300 Gy. It was fonud that there was no significant differences with respect to hatching ability between the control and irradiated groups of eggs in low doses such as 1, 5,10 and 20 Gy when larval development in eggs were examined. The results show that there is an inverse relatonship between the gamma radiation dose used and the hatching ability of eggs after the dose of 30 Gy. The more the radiation dose level, the lesser the hatching was observed and there was no hatchings after the dose of 225 Gy. Moreover, degenerative changes in the developments and multiplications of blastomrs relating to increases of radiation doses were observed. The differences between the control group and irradiated groups (starting from 30 Gy) were found to be statistically significant (P<0.05). Twelve Angora goats were infected with each receiving 500 Nematodirus sp. Larvae irradiated with doses of 0, 30, 50 and 100 Gy. All animals were sacrificed and worm counts were performed 28 days after infection. It was determined that there were decreases in the egg and worm counts and delayed egg sheddings with the increases of irradiation doses and no worms were obtained at a dose of 100 Gy. Also, the survival of more females than males in the collected parasites was found.
Description
Keywords
Radiation, Radyasyon, Nematodirus sp, Gama ışınlaması, Viability, Canlılık, Infectivity, Bulaşıcılığı, Nematodirus
Citation
Çerçi, H. ... [ve arkadaşları]. (2003). Effect of radiation on viability and infectivity of nematodirus SP. eggs. Turkish Journal of Nuclear Sciences, 29(1-2), 27-33.