The identification of granules observed under the phase-contrast microscope of cells in tissue culture
Loading...
Files
Date
1972
Authors
Journal Title
Journal ISSN
Volume Title
Publisher
T.C. Başbakanlık Atom Enerjisi Komisyonu, Çekmece Nükleer Araştırma ve Eğitim Merkezi
Abstract
Doku kültürü tekniği ile yetiştirilen normal ve anormal albino sıçan hücrelerinin faz-kontrast mikroskobu ile tetkiklerinde sitoplazmalarında bazı teşekküller görülmektedir. Hücreler vital olarak Janus yeşili ile boyandıktan sonra faz-kontrast mikroskobu ile tetkik edildi. Diğer gurup hücreler de Regaud fiksatifi ile fikse edilip Regaud demir he mat ok s il in i ile boyanıp tetkik edildiler. Tetkik edilen hücrelerin sitoplazmalarında görülen bu teşekküllerin bir kısmının mito- kondriumlar, bir kısmının da sitoplazmik diğer teşekküller olabileceği tespit edildi.
The presence of some structures in the cytoplasms of normal and abnormal cells of albino rats in tissue culture were found to exist when the cells were examined under phase-contrast microscope. The cells were examined under phase-contrast microscope after they were stained vitally with Janus green. Another group of cells were also examined after being fixed with Regaud iron hematoxylin. It was concluded that some of the structures observed in the cytoplasm of the cells with phase-contrast microscope were mitochondria and the rest of them were thought to be different kinds of structures.
The presence of some structures in the cytoplasms of normal and abnormal cells of albino rats in tissue culture were found to exist when the cells were examined under phase-contrast microscope. The cells were examined under phase-contrast microscope after they were stained vitally with Janus green. Another group of cells were also examined after being fixed with Regaud iron hematoxylin. It was concluded that some of the structures observed in the cytoplasm of the cells with phase-contrast microscope were mitochondria and the rest of them were thought to be different kinds of structures.
Description
TENMAK D.N.. 10318
Keywords
Tissue culture, Doku kültürü
Citation
Üçer, E. (1972). The identification of granules observed under the phase-contrast microscope of cells in tissue culture. İstanbul : T.C. Başbakanlık Atom Enerjisi Komisyonu, Çekmece Nükleer Araştırma ve Eğitim Merkezi.