Purification of bovine ferritin
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Date
1983-12
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Publisher
Turkish Atomic Energy Authority
Abstract
Yaklaşık molekül ağırlığı 450.000 olan yüksek molekül ağırlıklı demir depo proteini ferritin sığır dalağından ayrıldı. Çözeltilerde ferritin varlığı protein yoğunluğuna oranla yüksek demir içerikli oluşu ile saptandı. Ayrılan ferritinin saflığı % 5.0'lik polyacrylamide jel elektroforezi ile gösterildi. Jellerde hem demir hem de protein için boyanan çoklu (multiple) ferritin bantları gözlendi.
The iron storage protein ferritin with a high molecular weight of about 450.000 was isolated from the spleen of cattle. Confirmation of ferritin in solutions was made by showing that it had a high content of iron relative to the protein concentration. The purity of the isolated ferritin was tested by 5.0 % polyacrylamide disc gel electrophoresis. In the gels multiple ferritin bands stained for both iron and protein were observed.
The iron storage protein ferritin with a high molecular weight of about 450.000 was isolated from the spleen of cattle. Confirmation of ferritin in solutions was made by showing that it had a high content of iron relative to the protein concentration. The purity of the isolated ferritin was tested by 5.0 % polyacrylamide disc gel electrophoresis. In the gels multiple ferritin bands stained for both iron and protein were observed.
Description
Keywords
Purification, Saflaştırma, Bovine ferritin, Sığır ferritini
Citation
Özcan, H. ve Çetinkaya, N. (1983). Purification of bovine ferritin. Turkish Journal of Nuclear Sciences, 10(2), 118-123.