Determination of effective mutagen dose for carrot (Daucus carota ssp. sativus var. atrorubens alef and D. carota) callus cultures

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dc.contributor.authorBüyükdinç, Damla Turan
dc.contributor.authorKantoğlu, K. Yaprak
dc.contributor.authorKarataş, Arzu
dc.contributor.authorİpek, Ahmet
dc.contributor.authorEllialtıoğlu, Ş. Şebnem
dc.contributor.authorID46500tr_TR
dc.contributor.departmentTAEK-SANAEMtr_TR
dc.date.accessioned2019-05-17T07:39:20Z
dc.date.available2019-05-17T07:39:20Z
dc.date.issued2019
dc.description.abstractMutation breeding is used for plant variety, to obtain high yield, earliness, resistance to pest and diseases and also to abiotic stress conditions. Gamma radiation application in physical mutagen applications has advantages on the ease of use, the high permeability and the ability to reach the target cells and the nontoxic and harmful effects. It expanses the present variation by creating a mutation with physical mutagenesis via Co60 irradiation applied to in vitro donor plant tissues, and provides selection of variations at the cellular level in desired properties. Carrot is an important vegetable crops and in demand by the local markets throughout the year. This study was carried out to obtain a database and to determine the Effective Mutagen Dose (EMD50) in the course of mutation breeding studies on purple and orange carrots. For this aim, 0, 5, 10, 20, 30, 40, 50 and 60 Gray (Gy) doses of Co60 (gamma-rays) were applied to carrots calli as a physical mutagen of 30 callus pieces (each 1 g) per dose. 4-5 weeks after irradiation, shoot development of callus were determined and EMD50 calculated by linear regression analyses. As a result of the research, it was determined that 8,36 Gy was an effective mutation dose for purple carrot and 23,605 Gy for orange carrots due to the regression analyses.tr_TR
dc.description.sponsorshipRecep Tayyip Erdoğan Üniversitesi, Doğa Bilimleri Fakültesi, Bahçe Bitkileri Bölümü, TAEK, RHTDB / AR-GE Projeleri Şubesi Uludağ Üniversitesi Ziraat Fakültesi Bahçe Bitkileri Bölümü, Ankara Üniversitesi Ziraat Fakültesi BAhçe Bitkileri Bölümütr_TR
dc.identifier.citationBüyükdinç, D. T. ... [ve arkadaşları]. (2004). Determination of effective mutagen dose for carrot (Daucus carota ssp. sativus var. atrorubens alef and D. carota) callus cultures. International Journal of Scientific and Technological Research, 5(3), 15-23.tr_TR
dc.identifier.endpage23tr_TR
dc.identifier.issn2422-8702tr_TR
dc.identifier.issue3tr_TR
dc.identifier.startpage15tr_TR
dc.identifier.urihttps://www.iiste.org/Journals/index.php/JSTR/article/view/46755/48285
dc.identifier.urihttp://kurumsalarsiv.tenmak.gov.tr/handle/20.500.12878/1282
dc.identifier.volume5tr_TR
dc.language.isoengtr_TR
dc.publisherAcademic Hosting & Event Management Solutionstr_TR
dc.relation.isversionof10.7176/JSTR/5-3-02tr_TR
dc.relation.journalInternational Journal of Scientific and Technological Researchtr_TR
dc.rightsinfo:eu-repo/semantics/openAccesstr_TR
dc.subjectCarrotstr_TR
dc.subjectHavuçlartr_TR
dc.subjectMutation breedingtr_TR
dc.subjectMutasyon ıslahıtr_TR
dc.subjectCo60tr_TR
dc.subjectKobalt 60tr_TR
dc.subjectGamma raystr_TR
dc.subjectGama ışınlarıtr_TR
dc.subjectEffective mutagen (EMD50)tr_TR
dc.subjectEtkili mutajen (EMD50)tr_TR
dc.subjectIn vitrotr_TR
dc.subjectCam tüptetr_TR
dc.titleDetermination of effective mutagen dose for carrot (Daucus carota ssp. sativus var. atrorubens alef and D. carota) callus culturestr_TR
dc.typearticletr_TR
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