Türkiye Enerji, Nükleer ve Maden Araştırma Kurumu Kurumsal Araştırma ArşiviBrowsing by All Authors "Bekis, R."

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Labeling of cholecystokinin with 99mTc and investigation of its radiopharmaceutical potential
(Turkish Atomic Energy Authority, 2000-10) Ertay, T.; Ünak, Perihan; Bekis, R.; Yurt, Fatma; Biber, F. Zümrüt; Durak, H.; 171211; Bölüm Yok
The aim of this study is to label CCK8 with Tc-99m and to investigate its radiopharmaceutical potential. CCK8 was labeled with Tc-99m using GH and DTPA as bifunctional chelating agents. Labeling efficiency was higher than 99 %. Complex was stable more than 5 hours. 37 MBq Tc-99m-GH-CCK8 or Tc-99m-DTPA-CCK8 was administered to rabbits intravenously for biodistribution experiments. Dynamic and static images were obtained from anterior projection using a Camstar XC/T gamma camera. For quantitative evaluation, regions of interest were drawn on organs and time-activity curves were generated. The highest accumulation occurred in brain within 10 and 30 minutes after injection. Renal and hepatobiliary excretion were observed. For biodistribution studies on rats, 13.7 MBq Tc-99m-GH-CCK8 or Tc-99mDTPA-CCK8 was administered by tail vein. Rats were sacrificed at 10th minute, 30th minute, 2nd hour and 5th hour and the organs were removed. The rat brains were dissected into their components. The activity/g tissue was calculated. The highest activity was in hypothalamus. Results demonstrated that Tc-99m CCK8 may be a promising radiopharmaceutical for CCK receptor expressing organs.