Labeling of cholecystokinin with 99mTc and investigation of its radiopharmaceutical potential
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Date
2000-10
Journal Title
Journal ISSN
Volume Title
Publisher
Turkish Atomic Energy Authority
Abstract
The aim of this study is to label CCK8 with Tc-99m and to investigate its radiopharmaceutical
potential. CCK8 was labeled with Tc-99m using GH and DTPA as bifunctional chelating
agents. Labeling efficiency was higher than 99 %. Complex was stable more than 5 hours. 37
MBq Tc-99m-GH-CCK8 or Tc-99m-DTPA-CCK8 was administered to rabbits intravenously
for biodistribution experiments. Dynamic and static images were obtained from anterior
projection using a Camstar XC/T gamma camera. For quantitative evaluation, regions of interest
were drawn on organs and time-activity curves were generated. The highest accumulation
occurred in brain within 10 and 30 minutes after injection. Renal and hepatobiliary excretion
were observed. For biodistribution studies on rats, 13.7 MBq Tc-99m-GH-CCK8 or Tc-99mDTPA-CCK8 was administered by tail vein. Rats were sacrificed at 10th minute, 30th minute, 2nd
hour and 5th hour and the organs were removed. The rat brains were dissected into their
components. The activity/g tissue was calculated. The highest activity was in hypothalamus.
Results demonstrated that Tc-99m CCK8 may be a promising radiopharmaceutical for CCK
receptor expressing organs.
Description
I. Avrasya Nükleer Bilimler ve Uygulamaları Konferansı : 23-27 Ekim 2000. İzmir, Türkiye.
Keywords
Tc-99m / Technetium-99m, Teknesyum-99m, Labeling of cholecystokinin with 99mTc, Kolesistokinin 99mTc ile etiketlenmesi, Radiopharmaceutical, Radyofarmasötik, Peptide, Peptit, Cholecystokinin, Kolesistokinin, CCK-8, Tc-99M-GH-CCK8, Tc-99M-DTPA-CCK8, DTPA, Biodistribution of CCK8, 99mTc
Citation
Ertay, T., (2000). Labeling of cholecystokinin with 99mTc and investigation of its radiopharmaceutical potential. I. Eurasia Conference on Nuclear Science and Its Application, Presentations, Vol 1, (s. 586-591). 23-27 October 2000. İzmir, Turkey.