Tea extracts protect normal lymphocytes but not leukemia cells from UV radiation-induced ROS production : An EPR spin trap study

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dc.contributor.authorTepe Çam, Semra
dc.contributor.authorPolat, Mustafa
dc.contributor.authorEsmekaya, Meriç Arda
dc.contributor.authorCanseven, Ayşe G.
dc.contributor.authorSeyhan, Nesrin
dc.contributor.departmentTAEK-SANAEMtr_TR
dc.date.accessioned2017-02-17T08:41:50Z
dc.date.available2017-02-17T08:41:50Z
dc.date.issued2014-09
dc.description.abstractPurpose: An ex vivo method for detection of free radicals and their neutralization by aqueous tea in human normal lymphocytes and MEC-1 leukemia cells under ultraviolet (UV) irradiation was investigated. Materials and Methods: This method is based on the electron paramagnetic resonance (EPR) spectroscopy spin-trapping technique. 5-tert-butoxycarbonyl 5-methyl-1-pyrroline N-oxide (BMPO) was used as the spin trap. Normal human lymphocytes and leukemia cells were exposed to UVB radiation (290 to 315 nm) at 47.7 and 159 mJ/cm2 and to UVA radiation (315 to 400 nm) at 53.7 J/cm2. Results: No significant radical production at 47.7 mJ/cm2 UVB dose in both cell lines was observed. In normal cells, free radical production was observed at 159 mJ/cm2 UVB and 53.7 J/cm2 UVA doses. However, both UV sources did not significantly produce free radicals in leukemia cells. A radical scavenging property of tea extracts (black, green, sage, rosehip) was observed in normal lymphocytes after both UVB and UVA exposure. In leukemia cells, the intensities of EPR signals produced in BMPO with tea extracts were found to be increased substantially after UVA exposure. Conclusion: These results showed that UV radiation induced free radical formation in normal human lymphocytes and indicated that tea extracts may be useful as photoprotective agents for them. On the other hand, tea extracts facilitated free radical production in leukemia cells.tr_TR
dc.identifier.citationTepe Çam, S., Polat, M., Esmekaya, M.A., Canseven, A. G. ve Seyhan, N. (2014) Tea extracts protect normal lymphocytes but not leukemia cells from UV radiation-induced ROS production : An EPR spin trap study. 26. Ulusal Biyofizik Kongresi, 9-12 Eylül 2014. Tokat : Gaziosmanpaşa Üniversitesitr_TR
dc.identifier.urihttp://kurumsalarsiv.tenmak.gov.tr/handle/20.500.12878/287
dc.language.isoengtr_TR
dc.publisherTürk Biyofizik Derneğitr_TR
dc.relation.journal26. Ulusal Biyofizik Kongresi, Tokat, 9-12 Eylül 2014, Gaziosmanpaşa Üniversitesitr_TR
dc.rightsinfo:eu-repo/semantics/openAccesstr_TR
dc.subjectLymphocytetr_TR
dc.subjectLenfosittr_TR
dc.subjectLeukemiatr_TR
dc.subjectLösemitr_TR
dc.subjectEPR Spin traptr_TR
dc.subjectEPR Spin tuzağıtr_TR
dc.subjectRadicalstr_TR
dc.subjectRadikallertr_TR
dc.subjectAntioxidantstr_TR
dc.subjectAntioksidanlartr_TR
dc.subjectTea extracttr_TR
dc.subjectÇay özütr_TR
dc.subjectUVtr_TR
dc.titleTea extracts protect normal lymphocytes but not leukemia cells from UV radiation-induced ROS production : An EPR spin trap studytr_TR
dc.typeconferenceObjecttr_TR
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