Determination of biological nitrogen fixation capacities of winter and spring lentil varieties by using 15N methodology
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Date
2000-10
Authors
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Publisher
Turkish Atomic Energy Authority
Abstract
In order to determine the biological nitrogen fixation capacities of winter and spring varieties of
lentil which have of agronomic importance under the Central Anatolia region, the field
experiments (winter and spring) were carried out. In both experiments, the effects of two
different inoculants and different harvesting times on the biological nitrogen fixation capacities
of lentil varieties, were investigated.
The field experiments were conducted using by randomized block design as split-split plot for 4
replications. Barley was selected as the reference crop and 20 cm row spacing were used for
lentil and barley. Inoculations were done immediately before sowing. 10.0 kgN/ ha for lentil
varieties as 10.0 % 15N atom excess and 40.0 kgN/ ha for barley (reference crop) as 2.0 % 15N
atom excess ammonium sulphate fertilizer were applied. In addition, 60.0 kgP20 5/ ha were
applied as triplesuperphosphate for all treatments. Plants were harvested at the different growth
stages and than plant materials prepared for the analysis. Total nitrogen and % 15N atom excess
analysis were done by Kjeldahl method and Emission spectrometer, respectively. The amount of
nitrogen fixation capacities of winter and spring lentil varieties were calculated according to the
A-Value method (IAEA 1990).
The results showed us that the winter varieties of lentil had higher dry matter yields and
nitrogen fixation capacities than the spring varieties. Inoculation treatments had no statistically
significant effects on the percentage of nitrogen derived from atmosphere (% Ndfa) and the
amount of fixed nitrogen (kgN/ ha) for both experiments. In comparison between the harvesting
times, the highest amount of fixed nitrogen was found at the pod formation stage for all
cultivars. The average amounts of % Ndfa and fixed nitrogen (kgN/ ha) were 75.0 and 70.0 for
winter cultivars, 70.0 and 45.0 for spring cultivars, respectively.
Description
I. Avrasya Nükleer Bilimler ve Uygulamaları Konferansı : 23-27 Ekim 2000. İzmir, Türkiye.
Keywords
Biological nitrogen fixation capacities, Biyolojik azot fiksasyon kapasiteleri, Determination, Belirleme, Lentil varieties, Mercimek çeşitleri, 15N methodology, 15N metodoloji
Citation
Akın, A., (2000). Determination of biological nitrogen fixation capacities of winter and spring lentil varieties by using 15N methodology. I. Eurasia Conference on Nuclear Science and Its Application, Presentations, Vol 1, (s. 458-462). 23-27 October 2000. İzmir, Turkey.