Effects of Two Different Gamma Ray Sources on In Vitro Shoot Explantation of Fraser Photinia and Determination of Effective Mutation Dose
| dc.authorid | 0000-0002-3760-2801 | |
| dc.authorid | 0000-0002-7247-9116 | |
| dc.authorid | 0000-0002-3851-466X | |
| dc.contributor.author | Türkmen, Onur Sinan | |
| dc.contributor.author | Kantoğlu, K. Yaprak | |
| dc.contributor.author | Ellialtıoğlu, Ş. Şebnem | |
| dc.date.accessioned | 2025-01-15T10:53:08Z | |
| dc.date.available | 2025-01-15T10:53:08Z | |
| dc.date.issued | 2024-12-03 | |
| dc.department | TENMAK-Nükleer Enerji Araştırma Enstitüsü-Ankara | |
| dc.description.abstract | Fraser photinia (Photinia × fraseri cv. Red Robin) is widely preferred as ornamental plant in garden designs due to environmental resilience and decorative feature. Considering the increasing adaptation capacity of the existing varieties of the species to changing climatic conditions, efforts to develop new varieties with high attractiveness to the market have started to increase. The in vitro mutation breeding method has serious potential for expanding the existing variability for this species, which is prone to in vitro propagation. Determining the applicability of ionizing gamma ray sources for in vitro mutation studies on Fraser photinia is crucial. In this study, two different gamma-ionizing radiation sources cesium 137 (137Cs) and cobalt 60 (60Co) were used to determine the radiation sensitivity of in vitro shoot explants, and effective mutation dose (EMD50) values were determined for the sources. For this purpose, in vitro shoot explants were irradiated at thirteen different doses ranging from thirteen different doses (0, 10, 20, 30, 40, 50, 60, 70, 80, 90, 100, 110, 130 Gy) with 60Co (Dose rate: 235 Gy/h) and 137Cs (Dose rate: 821 Gy/h) gamma ray sources. Thirty days after irradiation, shoot length and number of leaves were measured in in vitro plantlets, and EMD50 values were calculated by linear regression analysis. The EMD50 dose based on the number of leaves as a result of cesium-137 source ray application was determined as 60.34 Gy, while this value was determined as 80.88 Gy for cobalt- 60 source. According to these results, it was seen that the source power, irradiation time, and the effect created by the linear energy transfer value of the beam during tissue penetration were effective on the EMD50 dose difference. | |
| dc.identifier.uri | https://kurumsalarsiv.tenmak.gov.tr/handle/20.500.12878/2067 | |
| dc.institutionauthor | Kantoğlu, K. Yaprak | |
| dc.language.iso | en | |
| dc.publisher | Türkiye Enerji, Nükleer ve Maden Araştırma Kurumu (TENMAK) | |
| dc.rights | info:eu-repo/semantics/openAccess | |
| dc.subject | EMD50 | |
| dc.subject | in vitro mutation | |
| dc.subject | Photinia fraseri | |
| dc.subject | Red Robin | |
| dc.title | Effects of Two Different Gamma Ray Sources on In Vitro Shoot Explantation of Fraser Photinia and Determination of Effective Mutation Dose | |
| dc.type | other |