Clinical evaluation of GLY-GLY-Alanine and same group peptides labeled with 99mTc

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dc.contributor.advisor99mTc
dc.contributor.authorTaner, Memduh Sami
dc.contributor.authorÖzdemir, Durmuş
dc.contributor.authorDuman, Yusuf
dc.contributor.departmentBölüm Yoktr_TR
dc.date.accessioned2018-07-13T13:00:42Z
dc.date.available2018-07-13T13:00:42Z
dc.date.issued2000-10
dc.descriptionI. Avrasya Nükleer Bilimler ve Uygulamaları Konferansı : 23-27 Ekim 2000. İzmir, Türkiye.tr_TR
dc.description.abstractIn this study, seven different (six of them with -N H and one of them with -NHSH side chain) peptides (Gly-l-Hystidine, Gly-l-Methionine, Gly-l-Tyrosine, Gly-Gly-l-Alanine, Gly-l-leucine amide, Gly-l-Glutamic acid, Gly-l-Gly-amide) were selected. For the positive control, tetraglycin (Gly-Gly-Gly-Gly) was chosen and for each of them, " mTc labeling studies have been performed using both direct and ligand exchange methods. Among these peptides, two of them (Gly-Gly-l-Alanine (GGA) and Gly-l-leucyl amide(GLA)) were labeled with the yields that were close to the USP acceptance criterions. Quality control has been performed by reverse phase HPLC analysis method with the use of a Beckman-Gold Radio-HPLC system. Latter on, radiopharmaceutical distribution and organ uptake characteristics of these peptides (" mTc-GGA and 99mTc-GLA) on rabbits were studied by using gamma camera imaging technique. Also, animal bio-distribution with GGA and inflammation imaging studies with GLA on rat models with sterile inflammation have been performed. Results indicated that in direct radio-labeling method developed for the small molecule peptides, labeling yields and retention times for " mTcGGA and " mTc-GLA radio-complexes were % 75.09 at 12 minute and % 74.20 at 17.5 minutes, respectively in reverse phase Radio-HPLC analysis. For the rest of the five peptides, direct and ligand exchange labeling methods were unsuccessful to produce a radio-labeled peptide that has an acceptable yield in terms of the radiopharmaceutical quality. Gamma camera imaging studies have indicated that both of the peptides ( " mTc-GGA and " mTcGLA) have demonstrated remarkable rapid blood clearance and dynamic discharge through kidneys. It is possible to say that based on the initial images and renogram data, dynamic kidney imaging can be obtained right after 30 minutes of the application and static kidney scintigrams can be taken by performing late imaging with " mTc-GGA radio-peptide complex. On the other hand, " mTc-GLA complex may have a potential use especially for very clear dynamic kidney and infection imaging studies. In bio-distribution study on rabbits, in terms of radio-peptide retention, the most important organ and body fluid samples were taken and then counted (first with collimated and then with non-collimated gamma camera counting system) with TENELLEC well type detector system. When compared to the other body organs, it was observed that kidneys showed the most active retention and the most of the applied radiopeptide were thrown out through urine. Also, In the infection imaging studies on mouse models with colitis using the same radio-labeled peptides, 99mTc-GLA proved to be more appropriate as indicated by the scintigrams.tr_TR
dc.identifier.citationTaner, M. S., Özdemir, D., Duman, Y., (2000). Clinical evaluation of GLY-GLY-Alanine and same group peptides labeled with 99mTc. I. Eurasia Conference on Nuclear Science and Its Application, Presentations, Vol 1, (s. 434-441). 23-27 October 2000. İzmir, Turkey.tr_TR
dc.identifier.endpage441tr_TR
dc.identifier.startpage434tr_TR
dc.identifier.urihttp://kurumsalarsiv.tenmak.gov.tr/handle/20.500.12878/725
dc.language.isoengtr_TR
dc.publisherTurkish Atomic Energy Authoritytr_TR
dc.relation.journalI. Eurasia Conference on Nuclear Science and Its Application : Presentations, 23-27 October 2000. İzmir, Turkey.tr_TR
dc.rightsinfo:eu-repo/semantics/openAccesstr_TR
dc.subjectGly-Gly-Alanine
dc.subjectClinical evaluationtr_TR
dc.subjectKlinik değerlendirmetr_TR
dc.subjectPeptides labeledtr_TR
dc.subjectEtiketli peptitlertr_TR
dc.subjectTc-99m / Technetium-99mtr_TR
dc.subjectTeknesyum-99mtr_TR
dc.titleClinical evaluation of GLY-GLY-Alanine and same group peptides labeled with 99mTctr_TR
dc.typeconferenceObjecttr_TR
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I. Eurasia conference on nuclear science and its application : presentations, 23-27 October 2000. İzmir, Turkey.