Gamma rays induced enhancement in the phytonutrient capacities of tomato (Solanum Lycopersicum L.)
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Date
2023-08-03
Journal Title
Journal ISSN
Volume Title
Publisher
Frontiers
Abstract
One of the most important problems for the field-grown Ayaş tomato (Solanum
lycopersicum Ayas population), which is preferred for consumption in Central
Anatolia because of its aromatic taste, is that the shelf life is short because of
insufficient fruit firmness. Therefore, a study was initiated to develop high-quality
lines and variety candidates through mutation breeding in the current Ayaş
population. In this study, the effective mutation dose (EMD50) was found to be
150 Gy for seeds using a Cobalt-60 gamma ray source. The main mutant population
was generated by applying EMD50. During the study, mutant lines selected by
following the classicalmutation breeding stages were evaluated in terms of yield and
quality traits (antioxidant content, total soluble solid amount, fruit firmness, Brix, etc.)
starting from the M4 stage. One of our aims was to determine and improve the
phytonutrient content of field-grown tomatoes. The fruits of selected lines at the M4
stage were extracted by liquid-liquid partition and accelerated solvent extraction
techniques and analyzed by High Performance Liquid Chromatography (HPLC) in
this study. Itwas found that 28 of the 29mutant lines were significantly different from
the control and mutant lines. Mutant lines 9-22, 8-90, 8-135, and 8-127 were
determined to be the most promising for commercialization.