Development of Petal Culture Method in Kalanchoe (Kalanchoe blossfeldiana Poelnn.) and Investigation of its Potential Use in In Vitro Mutation Breeding Studies
| dc.authorid | 0000-0002-7247-9116 | |
| dc.contributor.author | Kantoğlu Kadriye Yaprak | |
| dc.contributor.author | Sarıtoprak Okan | |
| dc.contributor.author | Akyüz Çağdaş Ebru | |
| dc.contributor.author | Okutan Evrim | |
| dc.contributor.author | Aktaş Hakan | |
| dc.contributor.author | Ellialtıoğlu Şeküre Şebnem | |
| dc.date.accessioned | 2025-11-03T06:41:29Z | |
| dc.date.available | 2025-11-03T06:41:29Z | |
| dc.date.issued | 2025-07-01 | |
| dc.department | TENMAK-Nükleer Enerji Araştırma Enstitüsü-Ankara | |
| dc.description.abstract | Kalanchoe (Kalanchoe blossfeldiana Poelnn.), is an important potted indoor plant as well as an outdoor ornamental plant and cut flower in recent years. Studies are being carried out using different breeding methods in order to develop new varieties suitable for market needs. In vivo and in vitro mutation breeding studies are important for creating different genetic variations for this species, which is highly prone to mutation breeding. In in vitro mutation applications, vegetative propagation methods are of critical importance in mass propagation of mutant single individuals while preserving genetic stability. This study consists of two stages: First, development and optimization of petal culture method in kalanchoe to provide in vitro vegetative propagation of mutant individuals, and second, morphological observation of genetic stability in clones obtained by propagation via petal culture from M1V4 kalanchoe mutant single plants obtained by using ionizing radiation. In vitro petal culture conditions were determined for kalanchoe and it was determined that Murashige and Skoog (MS) nutrient medium containing 2.0 mg L-1 thidiazuron (TDZ), 0.5 mg L-1 1-naphthalenacetic acid (NAA), 30 g L-1 sucrose, 6 g L-1 agar and pH 5.7 provided the best regeneration. In addition, after in vitro physical mutagen application, flowers were observed in mutant individuals propagated up to M1V4 stage in laboratory conditions and transferred to external conditions. Petals from plants with 4 different mutant flowers selected from these were cultured and propagated in vitro. As control, petals from a commercial variety were used for micropropagation. The flowers of the clones obtained showed homogeneity depending on whether the mutant flowers used as starting material were homogeneous or chimeric in appearance. Following this study, in which the first findings on petal culture in kalanchoe were obtained, studies are continuing to develop it comprehensively. | |
| dc.identifier.doi | 10.53518/mjavl.1552958 | |
| dc.identifier.eissn | 1694-7932 | |
| dc.identifier.endpage | 75 | |
| dc.identifier.issue | 1 | |
| dc.identifier.startpage | 62 | |
| dc.identifier.uri | https://kurumsalarsiv.tenmak.gov.tr/handle/20.500.12878/2084 | |
| dc.identifier.volume | 15 | |
| dc.language.iso | en | |
| dc.relation.journal | Manas Journal of Agriculture Veterinary and Life Sciences | |
| dc.rights | info:eu-repo/semantics/openAccess | |
| dc.rights | CC0 1.0 Universal | en |
| dc.rights.uri | http://creativecommons.org/publicdomain/zero/1.0/ | |
| dc.title | Development of Petal Culture Method in Kalanchoe (Kalanchoe blossfeldiana Poelnn.) and Investigation of its Potential Use in In Vitro Mutation Breeding Studies | |
| dc.type | article |