İyonlaştırıcı ışınların in vivo ve in vitro serum albuminine etkisi
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Date
1984-03
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Türkiye Atom Enerjisi Kurumu, Çekmece Nükleer Araştırma ve Eğitim Merkezi
Abstract
İyonlaştırıcı ışınların serum albuminine etkisi in vivo ve iv vitro deneylerle araştırıldı.
İn vivo deneylerde erkek sıçanlar(Rattus norvegicus) 225 röntgen bütün-vücut X-ışınlamasına tutuldu.Radyasyonun zamana bağlı olarak albumin seviyesine etkisi ışınlamadan hemen, 2.5 saat ve üç gün sonra incelendi.Albumin seviyesi ışınlamadan 2.5 saat sonra kontrol seviyesinin altına düştü, ışınlamadan üç gün sonra ise kontrol seviyesine ulaştı.Pre-albumin/albumin oranında ışınlamadan sonra artma oldu.
İn vitro deneylerde yüzde 0.5 sulu albumin çözeltisi 0.2, 0.5, 1.0 ve 1.9 Mrad dozlarda 60Co gamma ışınlamasına tutuldu.Deneyler serum albuminin elektroforetek mobilitesinin ışınlamadan sonra a- zaldığını gösterdi.Serum albuminin 0.2,0.5,1.0 ve 1.9 Mrad dozlarda in vitro ışınlanması UV absorpsiyon spektrumunda bir değişiklik meydana getirmedi.Serum albümini ışınlamadan sonra suda daha az çözünür hale geldi .Radyasyon albumin molekülünde agregatlar ve bölünme ürünleri meydana getirdi.
The effect of ionizing radiations on rat serum albumin was studied on in vivo and in vitro. Male rats(Rattus norvegicus) were exposed to 225 roentgen whole-body X-irradiation on in vivo experiments. Time-course effects of irradiation on albumin level examined at immediately,2.5 hours and 3 days after irradiation. Albumin level decreased above control level 2.5 hours after irradiation and rised within 3 days reaching contr- rol level.Pre-albumin/albumin ratio enhanced after X-irradiation. Aqueous solutions(0.5 percent) of rat serum albumin was exposed to various doses(0.2,0.5,1.0 and 1.9 Mrad) of 60Co gamma irradiation on in vitro experiments.Results showed that electrophoretic mobility of serum albumin decreased after gamma irradiation.Ro significant change in albumin UV absorption spectrum was observed at 0.2,0.5,1.0 and 1.9 Mrad doses.Albumin becomes progresively less soluble in water as the radiation doses is increased.Radiation induced transformation into insoluble albumin agregates and scission products.
The effect of ionizing radiations on rat serum albumin was studied on in vivo and in vitro. Male rats(Rattus norvegicus) were exposed to 225 roentgen whole-body X-irradiation on in vivo experiments. Time-course effects of irradiation on albumin level examined at immediately,2.5 hours and 3 days after irradiation. Albumin level decreased above control level 2.5 hours after irradiation and rised within 3 days reaching contr- rol level.Pre-albumin/albumin ratio enhanced after X-irradiation. Aqueous solutions(0.5 percent) of rat serum albumin was exposed to various doses(0.2,0.5,1.0 and 1.9 Mrad) of 60Co gamma irradiation on in vitro experiments.Results showed that electrophoretic mobility of serum albumin decreased after gamma irradiation.Ro significant change in albumin UV absorption spectrum was observed at 0.2,0.5,1.0 and 1.9 Mrad doses.Albumin becomes progresively less soluble in water as the radiation doses is increased.Radiation induced transformation into insoluble albumin agregates and scission products.
Description
TENMAK D.N. 10258
Keywords
Ionizing radiation, İyonlaştırıcı radyasyon, Iv vitro, X-rays, In vivo, X-ışınları
Citation
Portakal, S. (1984). İyonlaştırıcı ışınların in vivo ve in vitro serum albuminine etkisi. İstanbul : Türkiye Atom Enerjisi Kurumu, Çekmece Nükleer Araştırma ve Eğitim Merkezi.