Browsing by All Authors "Yurt, Fatma"
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Item An investigation on radiopharmaceutical potantial of I-131 labeled sertraline(Turkish Atomic Energy Authority, 2000-10) Yurt, Fatma; Ünak, Perihan; Biber, F. Zümrüt; 10811; Bölüm YokSertraline [(IS- cis)- 4-( 3, 4-dichlorophenyl)-l,2,3,4 -tetrahydro - N-methyl-l-naphalenamine hydrochloride] is an antidepressant drug. It is presumed to be linked to its inhibition of central nervous system (CNS) neuronal uptake of serotonin (5-HT) receptors. Serotonin 5-HT1A receptors are prominently expressed in the CNS that binding sites are present various brain regions. The aim of this study is to label with 1-131 and investigate of its radiopharmaceutical potential. Sertraline was labeled with 1-131 by using iodogen method. Quality Controls were fixed by Radio-TLC and Electrophoresis Methods. Labeling yield was 85-90% and spesific activity was approximately 1.75 Ci/mmol. The purification of radioiodinated Sertraline was performed by Sep Pak C-18 plus and determined radiochemical purity over 99%. Biodistribution studies were carried out by male Albino Wistar rats. 1-131 labeled Sertraline was administered by intravenous injection into tail vein of the rats. The rats were sacrificed by ether narcotization at certain time intervals and the organs were removed. The whole brain was excised quickly and dissected into their regions. Their activities were counted by Cd(Te) detector equipped with RAD 501 Single Channel Analyzer Instrument. The percentage injected radioactivity per gram of tissue was calculated, and these data versus time curves were generated for organs and brain regions. Obtained results showed that 1-131 labeled Sertraline may be a promising radiopharmaceutical for investigation of serotonin 5-HT receptor functions of brain.Item Determination of iodine by isotope dilution analysis in food and biological medium(Turkish Atomic Energy Authority, 2000-10) Ünak, Perihan; Yurt, Fatma; Biber, F. Zümrüt; Bölüm YokIodine is an important trace element and is one of main constituent of thyroid hormones. In this study, a method was developed for which can be applied routine iodide analysis in environmental and biological samples. The method based on substochiometric isotope dilution analysis (IDA). Iodide concentrations have been measured in several media such as urine, drinking water, milk and commercial salts.Item Labeling of chlorodiazepoxide with I-131 and biodistribution studies on rats(Turkish Atomic Energy Authority, 2000-10) Ünak, Perihan; Yurt, Fatma; Biber, F. Zümrüt; 11752; Bölüm YokChlordiazepoxide (CDZ) was labeled with I-131 and investigated its radiopharmaceutical potential as a benzodiazepine receptor agent. Iodogen was used as iodination agent. Labeling yields were determined by radio-ITLC (Instant Thin Layer Chromatography) and Paper Electrophoresis. Labeling yield was approximately 90%. Purification were performed by SepPak C-18 plus. Biodistribution studies were carried out by Albino Wistar rats. I-131 labeled Chlordiazepoxide which is the 62.2 GBq/mmol specific activity was administered by tail vein into the rats. Rats were sacrificed by ether narcotization at certain time intervals and the organs were removed. The brains were dissected into their components. Their activities were counted by a gamma counter. The activity per gram tissue was calculated and time activity curves were generatedItem Labeling of cholecystokinin with 99mTc and investigation of its radiopharmaceutical potential(Turkish Atomic Energy Authority, 2000-10) Ertay, T.; Ünak, Perihan; Bekis, R.; Yurt, Fatma; Biber, F. Zümrüt; Durak, H.; 171211; Bölüm YokThe aim of this study is to label CCK8 with Tc-99m and to investigate its radiopharmaceutical potential. CCK8 was labeled with Tc-99m using GH and DTPA as bifunctional chelating agents. Labeling efficiency was higher than 99 %. Complex was stable more than 5 hours. 37 MBq Tc-99m-GH-CCK8 or Tc-99m-DTPA-CCK8 was administered to rabbits intravenously for biodistribution experiments. Dynamic and static images were obtained from anterior projection using a Camstar XC/T gamma camera. For quantitative evaluation, regions of interest were drawn on organs and time-activity curves were generated. The highest accumulation occurred in brain within 10 and 30 minutes after injection. Renal and hepatobiliary excretion were observed. For biodistribution studies on rats, 13.7 MBq Tc-99m-GH-CCK8 or Tc-99mDTPA-CCK8 was administered by tail vein. Rats were sacrificed at 10th minute, 30th minute, 2nd hour and 5th hour and the organs were removed. The rat brains were dissected into their components. The activity/g tissue was calculated. The highest activity was in hypothalamus. Results demonstrated that Tc-99m CCK8 may be a promising radiopharmaceutical for CCK receptor expressing organs.Item Preparing an estrogen-derivative compound labeled with Tc-99m and determination of radiopharmaceutical potential(Turkish Atomic Energy Authority, 2000-10) Enginar, Hüseyin; Ünak, Perihan; Yurt, Fatma; Biber, F. Zümrüt; 58588; Bölüm YokIn recent years, estrogen derivative compounds have been used with several purpose which may lead to rapid advances in both diagnosis and therapy of some human cancers. In this study, an estrogen derivative compound (ESTCPTA) that 3,17-( β-estradiolyl propinol attached with 1-(4-methylbenzoic acid)-4,8,11- tetraazacyclotetradecane (CPTA) was synthesized at five steps. Synthesized product was purified by recrystallization in ethyl alcohol. Characterizations of purified products were made by NMR and IR spectroscopy. Synthesized compound was labeled with Tc-99m. RTLC (Radio Thin Layer Chromatography) and radiopaper electrophoresis were used to determine radiochemical yields. Specific activity was approximately 23.7 GBq/mmol and labeling yield was over 95%. Biodistribution studies were performed on female Albino Wistar rats. Rats were sacricified by ether narcotization at certain time intervals and the organs were removed. Their activities were counted by a gamma counter. The activity per gram tissue was calculated and time activity curves were generated.Item Stability of iodine content in iodized salt(Turkish Atomic Energy Authority, 2000-10) Biber, F. Zümrüt; Ünak, Perihan; Yurt, Fatma; 34687; Bölüm YokStabilization of iodine in salt have been determined by using Isotope Dilution Analysis. Heating, heating with oxidizing agent, incubation by time was the parameters which have been tried. Over 80% of iodide were lost during the treatment with oxidizing agent in heat. In the case of heating without an oxidation agent, loss of iodine was fewer.